1. Department of Medical Chemistry and Biochemistry, Medical University-Sofia, Sofia, Bulgaria
2. Pathophysiology of dementia, German Centre for Neurodegenerative Diseases, Magdeburg, Germany;
3. Department of Anatomy, Medical Univeresity-Sofia, Sofia , Bulgaria
Many evidences were shown last years, that mesenchymal tissues contain a reservoir of postnatal stem cells. We assessed the differentiation towards ectodermal, mesodermal and endodermal lineage of mesenchymal cell cultures from deciduous tooth pulp. Stem cells from human exfoliated deciduous teeth (SHED) were isolated and expanded in DMEM containing 10% FBS in vitro.
For odontogenic differentiation DMEM was supplemented 10 μM Dexamethasone, 100μM Ascorbic acid, 10mM β-glycerophosphate. Adipogenic differentiation medium contained DMEM supplemented with Dexamethasone and Indomethacine. For hepatic differentiation the supplements of DMEM were as follows – hepatocite growth factor (HGF), dexamethasone and oncostatin.
After odontogenic differentiation we stained the cells with Alizarin Red. The staining revealed Ca+2 deposits (red) in SHED cultures. Odontogenically differentiated cells also expressed p21 and alkaline phosphatase. Oil Red-O staining revealed fatty droplets in the cytoplasm of the cells, after adipogenic differentiation. The cultures proved to have significant amount of AFP and albumin positive cells after 20 days of hepatic differentiation. Differentiated cell cultures contained small clusters of IGF-1
The present results demonstrate multilineage potential and the ability of SHED to differentiate to odontoblasts, adipocytes and hepatocytes…